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Target | Aortic Smooth Muscle Cells (ASMC) |
Origin | Rabbit |
Host | Rabbit |
Purity | Negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. |
Storage | Shipped at -70 °C. Upon receipt, store in liquid nitrogen (-196 °C). Avoid repeated freeze/thaw cycles. |
Validity | 12 months. |
Buffer | Contains 90% FBS and 10% DMSO. |
Biological Activity | Cell activity: > 85% (viability by Trypan Blue exclusion) |
Availability | Shipped within 20-30 working days. |
Note | This product is for research use only. This product is shipped with dry ice. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Directions for use | Recommended Cell Culture Conditions: DMEM/F12 + 5% FBS + Rabbit Endothelial Cell Growth Supplement + 1% Penicillin-Streptomycin Solution @ 37 °C, 95% air, 5% CO2.Cell Recovery: Thaw cells in a 37 °C water bath with shaking until the mixture has dissolved. Transfer to a centrifuge tube and add culture medium (see Recommended Cell Culture Conditions above) at a volume 3-5 times the volume of the cells. Centrifuge at 1000 RPM for 5 minutes and discard the supernatant. Transfer to a T25 flask for culture. Suggested Cell Passage Procedure: Cells should be 85-95% confluent before cell passage is carried out.
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