25-Hydroxyvitamin D3 (Calcidiol) Antibody Pair

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Catalogue No: abx370873
Price: US$1,740.00
(Size: 5 × 96 tests)
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25-Hydroxyvitamin D3 (Calcidiol) Antibody Pair

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Datasheet SDS
25-Hydroxyvitamin D3 (Calcidiol) Antibody Pair for use in Competitive ELISA assay development. This antibody pair contains:

Component 5 × 96 tests 10 × 96 tests
Capture Antibody200 µg 400 µg
Biotin-Conjugated Competitor 50 µg 100 µg
Standard 2 µg 10 µg

Please note that quantities and concentrations may change between different batches.

It is recommended to use this antibody pair with abx098959 Antibody Pair Support Kit (Competitive Method).

Target 25-Hydroxyvitamin D3 (Calcidiol)
Reactivity General (All species)
Tested Applications ELISA
Recommended dilutions Dilute the Capture Antibody 125-fold with Coating Buffer.
Dilute the Biotin-Conjugated Competitor 200-fold with Biotin-Conjugated Competitor Diluent.

Optimal dilutions/concentrations should be determined by the end user.
Form Liquid (Capture Antibody and Biotin-Conjugated Competitor)
Reconstitution Reconstitute the standard with Standard Diluent. The volume, and therefore standard concentration, should be determined by the end user.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Buffer The Capture Antibody and Biotin-Conjugated Competitor both contain 0.1% sodium azide.
Test Range 246.9 pg/ml - 20000 pg/ml
Standard Form Lyophilized
Assay Type Competitive
Capture Antibody Host Mouse
Capture Antibody Clonality Monoclonal
Capture Antibody Conjugation Unconjugated
Detection Antibody Conjugation Biotin
Concentration Capture Antibody: 0.5 mg/ml
Biotin-Conjugated Competitor: 0.2 mg/ml
Availability Shipped within 5-20 working days.
Note This product is for research use only.
Directions for use Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. Working solutions should be prepared and used immediately.

Recommended Procedure:

  1. Dilute the Capture Antibody to working concentration using Coating Buffer. Immediately coat the 96-well plate with diluted Capture Antibody (100 µl per well). Seal the plate and incubate at 4 °C overnight or at 37 °C for 2 hours
  2. Aspirate the wells and wash with Wash Buffer (350 µl per well) and allow to soak for 1-2 min. Remove the liquid by inverting and tapping the plate on to absorbent paper.
  3. Block the plate with Blocking Buffer (200 µl per well) at 37 °C for 1.5 hours.
  4. Repeat the aspiration/wash process in Step 2.
  5. Add 50 µl of standards or sample into the appropriate wells, followed by 50 µl of working Biotin-Conjugated Competitor. Cover with a plate sealer and incubate at 37 °C for 1 hour.
  6. Repeat the aspiration/wash process in Step 2.
  7. Add appropriately diluted Streptavidin HRP (100 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 30 min.
  8. Repeat the aspiration/wash process in Step 2, for a total of 5 times.
  9. Add Substrate Solution (90 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 10-20 min. Keep the plate in the dark and avoid exposure to light.
  10. Add Stop Solution (50 µl per well). Tap the side of the plate to ensure thorough mixing.
  11. Measure the absorbance immediately using a microplate reader set at 450 nm.
Research Articles on 25-Hydroxyvitamin D3 (Calcidiol)


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