Actin Alpha 2, Smooth Muscle (ACTA2) Antibody

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Catalogue No: abx005470
Price: US$797.50
(Size: 96 tests)

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Western blot analysis of lysates from Mouse heart, using Alpha-Smooth Muscle Actin Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.
Actin Alpha 2, Smooth Muscle (ACTA2) Antibody Actin Alpha 2, Smooth Muscle (ACTA2) Antibody Actin Alpha 2, Smooth Muscle (ACTA2) Antibody Actin Alpha 2, Smooth Muscle (ACTA2) Antibody Actin Alpha 2, Smooth Muscle (ACTA2) Antibody Actin Alpha 2, Smooth Muscle (ACTA2) Antibody Actin Alpha 2, Smooth Muscle (ACTA2) Antibody Actin Alpha 2, Smooth Muscle (ACTA2) Antibody

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Datasheet SDS
ACTA2 Antibody is a Rabbit Polyclonal antibody against ACTA2. The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Target Actin Alpha 2, Smooth Muscle (ACTA2)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IHC-P: 1/20 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Alpha-Smooth Muscle Actin (ACTA2).
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P62736 (UniProt, ExPASy)
UniProt Secondary AC B2R8A4, P03996, P04108, Q6FI19
UniProt Entry Name ACTA_HUMAN
Gene Symbol ACTA2
GeneID 59
NCBI Accession NP_001604.1
KEGG hsa:59
String 9606.ENSP00000402373
Molecular Weight Calculated MW: 42 kDa
Observed MW: 42 kDa
Buffer PBS, pH 7.3, containing 0.09% sodium azide, 50% glycerol.
Concentration 0.35 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Actin Alpha 2, Smooth Muscle (ACTA2)
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