Actin Alpha 2, Smooth Muscle (ACTA2) Antibody

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Catalogue No: abx227123
Price: US$304.50
(Size: 40 µl)
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IHC-P analysis of alpha smooth muscle actin positivity in the vessel walls of submucosa of small intestine tissue (4 µm section).
Actin Alpha 2, Smooth Muscle (ACTA2) Antibody

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Datasheet SDS
Actin Alpha 2, Smooth Muscle (ACTA2) Antibody is a Rabbit Monoclonal antibody for the detection of Alpha Smooth Muscle Actin.

Target Actin Alpha 2, Smooth Muscle (ACTA2)
Clonality Monoclonal
Clone Z996
Reactivity Human, Mouse
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the N-terminal region of human alpha smooth muscle actin.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC P62736 (UniProt, ExPASy)
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use Suggested IHC-P Protocol
  1. Preparation of Tris-EDTA Buffer (10 mM Tris Base, 1 mM EDTA solution, 0.05% Tween-20, pH 9.0): Mix 1.21 g Tris and 0.37 g EDTA and dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1 M HCl and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  2. Preparation of Wash Buffer: Use PBS, pH 7.0-7.5, containing 0.05% Tween-20.
  3. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
  4. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  5. Rinse twice in distilled water, 5 minutes each.
  6. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  7. Wash twice in distilled water, 5 minutes each.
  8. Antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, and incubate in a water bath for 20 minutes at 95-97 °C.
  9. Remove the slide from the water bath and allow to stand at room temperature (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
  10. Rinse twice in distilled water, 5 minutes each.
  11. Wash twice in Wash Buffer, 5 minutes each.
  12. Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (> 1 mg/ml) to dilute this antibody.
  13. Wash 3 times with Wash Buffer, 5 minutes each.
  14. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  15. Wash 3 times in Wash Buffer, 5 minutes each.
  16. Apply the DAB chromagen for 1-3 minutes.
  17. Wash twice in water, 5 minutes each.
  18. Stain in hematoxylin for 5 minutes.
  19. Wash 3 times in water, 2 minutes each.
  20. Mount the slide for observation.
Research Articles on Actin Alpha 2, Smooth Muscle (ACTA2)


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