+44 (0) 1223 755950
+1 832 327 7413
Human Adiponectin Receptor 1 (ADIPOR1) Protein
REQUEST MORE INFO
Catalogue No: abx651293
Price:
US$551.00
(Size: 50 µg)
Reviews: 
(0 Publications and Reviews)
(0 Publications and Reviews)
Click on the image to see the image legend
Human Adiponectin Receptor 1 (ADIPOR1) Protein (Active) is an active Human protein.
Research Articles on Adiponectin Receptor 1 (ADIPOR1)
| Target | Adiponectin Receptor 1 (ADIPOR1) |
| Origin | Human |
| Tested Applications | WB, SDS-PAGE |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 98% |
| Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q96A54 (UniProt, ExPASy) |
| UniProt Secondary AC | B3KMB0, Q53HS7, Q53YY6, Q9Y360 |
| UniProt Entry Name | PAQR1_HUMAN |
| KEGG | hsa:51094 |
| String | 9606.ENSP00000341785 |
| Molecular Weight | Calculated MW: 65.4 kDa Observed MW: 63 kDa |
| Sequence Fragment | Met1-Gly136 |
| Sequence | MSSHKGSVVA QGNGAPASNR EADTVELAEL GPLLEEKGKR VIANPPKAEE EQTCPVPQEE EEEVRVLTLP LQAHHAMEKM EEFVYKVWEG RWRVIPYDVL PDWLKDNDYL LHGHRPPMPS FRACFKSIFR IHTETG |
| Tag | N-terminal His tag and MBP tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.05% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | Adiponectin Receptor 1 (ADIPOR1) is a receptor for 30 kDa adipocyte complement-related protein (ADP), an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Human ADP and rat ADP have very similar amino acid sequence with an identity of 83.2%, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ADIPOR1 and recombinant rat ADP. Briefly, ADIPOR1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to ADP-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-ADIPOR1 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of ADIPOR1 and ADP is shown in Figure 3. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |