AEC Chromogen Kit

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Catalogue No: abx097196
Price: US$232.00
(Size: 1 Kit)
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Immunohistochemical staining of human liver carcinoma and human colon carcinoma formalin-fixed paraffin-embedded tissue, using AEC Chromogen Kit. The section was then counterstained with hematoxylin and mounted with neutral gum.
AEC Chromogen Kit

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Datasheet SDS
AEC Chromogen Kit contains AEC (3-Amino-9-Ethylcarbazole), a widely used chromogen for immunohistochemical staining. AEC is a substrate for peroxidase and produces a red product that is soluble in alcohol and must be used with an aqueous counterstain and mounting media. It produces a clean, intense stain that is free of unwanted background.

Kit components:
  • PBS: 2 × 30 ml
  • Substrate Reagent: 3 ml
  • AEC Chromogen: 3 ml


Tested Applications WB, IHC, DB
Storage Store all components between 2-8 °C.
Buffer Contains AEC.
Availability Shipped within 7-12 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use
  1. Shortly before incubation, prepare the Working AEC Substrate Reagent by adding to a clean tube 1 ml PBS, 50 µl Substrate Reagent and 50 µl AEC Chromogen. Mix thoroughly.
  2. Apply 50-100 µl of Working AEC Substrate Reagent to each slide. Incubate for 5-10 minutes.
  3. When sufficient staining has been achieved, rinse slides in PBS for 3 minutes. Repeat this wash step for a total of 3 times.

Notes:
  • Do not use solutions containing alcohol for counterstaining, as the AEC stain is soluble in organic solvents.
  • The nitrocellulose strip will normally have a slight reddish background.
  • Use personal protective equipment (PPE) such as lab coats, gloves and goggles, when carrying out the assay.
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