The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t (9;11) (p22;q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified; BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.
Target |
ALL1-Fused Gene From Chromosome 9 Protein (MLLT3) |
Clonality |
Polyclonal |
Reactivity |
Human, Mouse |
Tested Applications |
ELISA, WB |
Host |
Rabbit |
Recommended dilutions |
WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation |
Unconjugated |
Immunogen |
KLH-conjugated synthetic peptide between 407-438 amino acids from the Central region of human AF9 (MLLT3). |
Isotype |
IgG |
Form |
Liquid |
Purification |
Purified Rabbit Polyclonal Antibody. |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC |
P42568 (UniProt,
ExPASy)
|
UniProt Secondary AC |
B1AMQ2, B2R7B3, B7Z755, D3DRJ8, Q8IVB0 |
UniProt Entry Name |
AF9_HUMAN |
KEGG |
hsa:4300
|
String |
9606.ENSP00000369695
|
Molecular Weight |
Calculated MW: 63.4 kDa |
Buffer |
PBS containing 0.09% sodium azide. |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |
Research Articles on ALL1-Fused Gene From Chromosome 9 Protein (MLLT3)