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Target | RAC-Alpha Serine/Threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) |
Clonality | Polyclonal |
Target Modification | Ser473 |
Modification | Phosphorylation |
Reactivity | Human, Mouse, Rat |
Tested Applications | WB, IHC, IF/ICC |
Host | Rabbit |
Recommended dilutions | WB: 1/500 - 1/3000, IHC: 1/50 - 1/200, IF/ICC: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of Serine 473 (Q-F-SP-Y-S). |
Isotype | IgG |
Form | Liquid |
Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. |
Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P31749 (UniProt, ExPASy) |
UniProt Secondary AC | B2RAM5, B7Z5R1, Q9BWB6 |
UniProt Entry Name | AKT1_HUMAN |
KEGG | hsa:207 |
String | 9606.ENSP00000451828 |
Enzyme Commission Number | EC 2.7.11.1 |
Sequence | CPHFPQFSYSASST |
Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
Specificity | Detects endogenous levels of Akt only when phosphorylated at Ser473. |
Concentration | 1 mg/ml |
Availability | Shipped within 5-10 working days. |
Note | This product is for research use only. |