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RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) Antibody
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Catalogue No: abx031983
Price:
US$326.25
(Size: 80 µl)
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The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Research Articles on RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473)
| Target | RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) |
| Clonality | Polyclonal |
| Target Modification | Ser473 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, WB, DB |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000, DB: 1/500. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S473 of human AKT1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein A affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with peptides. The antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P31749 (UniProt, ExPASy) |
| UniProt Secondary AC | B2RAM5, B7Z5R1, Q9BWB6 |
| UniProt Entry Name | AKT1_HUMAN |
| NCBI Accession | NP_001014431.1 NP_001014432.1 NP_005154.2 |
| KEGG | hsa:207 |
| String | 9606.ENSP00000451828 |
| Molecular Weight | Calculated MW: 55.7 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse AKT1. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |