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AKT1 / 2 / 3 Cell ELISA Kit
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Catalogue No: abx595960
Price:
US$572.75
(Size: 96 tests)
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AKT1/2/3 Cell ELISA Kit is a cell-based ELISA Kit. Cells to be assayed should be seeded onto a clear flat bottom 96 well plate, using poly-L-lysine for non-adherent cells. Cells should be grown to 75-90% confluence and treated prior to carrying out the ELISA.
Research Articles on AKT1/2/3
| Introduction | The RAC serine/threonine-protein kinases (AKT1, 2, and 3 in humans, also known as Protein Kinase B, PKB) are a group of enzymes that catalyse the phosphorylation of serine and/or threonine residues on proteins, using ATP as a phosphate donor. This allows them to regulate the activity of other proteins, which are chiefly involved in glucose metabolism, cell survival and proliferation, and migration. Overexpression of AKT proteins occurs in various tumours. |
| Target | AKT1/2/3 |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | 6 months. |
| UniProt Primary AC | P31749 (UniProt, ExPASy) P31751 (UniProt, ExPASy) Q9Y243 (UniProt, ExPASy) |
| UniProt Secondary AC | B2RAM5, B7Z5R1, Q9BWB6 |
| UniProt Entry Name | AKT1_HUMAN, AKT2_HUMAN, AKT3_HUMAN |
| Gene Symbol | AKT1, AKT2, AKT3 |
| GeneID | 207 208 10000 |
| OMIM | 164730 164731 611223 |
| Detection Method | Colorimetric |
| Assay Data | Qualitative |
| Plate Coating | Uncoated |
| Substrate | TMB |
| Detection Antibody Conjugation | HRP |
| Availability | Shipped within 1-2 weeks. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES. Please note that our kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |