Alkaline Phosphatase, Placental Type (ALPP) Antibody

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Catalogue No: abx227087
Price: US$232.00
(Size: 40 µl)
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IHC-P analysis of diffuse cytoplasmic PLAP positivity in the typical seminoma (4 µm section).
Alkaline Phosphatase, Placental Type (ALPP) Antibody

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Datasheet SDS
Alkaline Phosphatase, Placental Type (ALPP) Antibody is a Rabbit Monoclonal antibody for the detection of PLAP (Placental Alkaline Phosphatase).

Target Alkaline Phosphatase, Placental Type (ALPP)
Clonality Monoclonal
Clone H448
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the middle portion of human PLAP sequence.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC P05187 (UniProt, ExPASy)
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use Suggested IHC-P Protocol
  1. Preparation of Citrate Buffer (10 mM Citric Acid, 0.05% Tween-20, pH 6.0): Dissolve 1.92 g of anhydrous citric acid in 700 ml of distilled water. Adjust pH to 6.0 with 1 M NaOH and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  2. Preparation of Wash Buffer: Use 0.05 M Tris-HCl, pH 7.6, containing 0.2% Tween-20.
  3. Deparaffinize the section in 3 changes of xylene, 5 minutes each.
  4. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  5. Rinse in distilled water.
  6. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  7. Wash in distilled water for 5 minutes.
  8. Antigen retrieval: immerse the slide in Citrate buffer, pH 6.0, and incubate in a water bath for 30-40 minutes at 96-98 °C.
  9. Remove the slide from the water bath and allow to stand at room temperature (in Citrate buffer, pH 6.0) for 15 minutes.
  10. Rinse in distilled water.
  11. Wash in Wash Buffer for 5 minutes.
  12. Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (≥ 1 mg/ml) to dilute this antibody.
  13. Wash twice with Wash Buffer, 5 minutes each.
  14. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  15. Wash twice in Wash Buffer, 5 minutes each.
  16. Apply the DAB chromagen for 1-3 minutes.
  17. Rinse in water for 10 minutes.
  18. Stain in hematoxylin for 5 minutes.
  19. Wash in water for 10 minutes.
  20. Dehydrate the section in 2 changes of 96% ethanol, 5 minutes each.
  21. Wash the section in 2 changes of xylene, 2 minutes each.
  22. Mount the slide for observation.
Research Articles on Alkaline Phosphatase, Placental Type (ALPP)


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