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Annexin V (Biotin)
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Catalogue No: abx092323
Price:
US$333.50
(Size: 100 µg)
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Datasheet SDS
Annexin V is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. The Annexin V affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (Annexin V binding to PS is Ca2+ dependent). The assay combines Annexin V staining of PS membrane events with the staining of the cell nucleus with PI or AAD-7 to distinguish living cells from dead cells. Annexin V apoptosis detection is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner (cytoplasmic-facing) leaflet of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy.
This product contains Annexin V (Biotin) only and does not include 10X Binding Buffer, PI or 7-AAD reagents.
Research Articles on Annexin V
| Target | Annexin V |
| Reactivity | General (All species) |
| Conjugation | Biotin |
| Form | Lyophilized |
| Storage | Store at at -20°C in the dark. |
| Validity | 24 months |
| Buffer | Does not contain Sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |