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Annexin V (FITC) / 7-AAD Apoptosis Detection Kit
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Catalogue No: abx090603
Price:
US$493.00
(Size: 100 tests)
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Datasheet SDS
Annexin V is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. The Annexin V affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (Annexin V binding to PS is Ca2+ dependent). The assay combines Annexin V staining of PS membrane events with the staining of the cell nucleus with PI or AAD-7 to distinguish living cells from dead cells. Annexin V apoptosis detection is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner (cytoplasmic-facing) leaflet of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy.This kit contains:
Research Articles on Annexin V
| Component | 100 tests | 200 tests |
| Annexin V (FITC) | 500 µl | 1 ml |
| Annexin V Binding Buffer (10X) | 11 ml | 2 × 11 ml |
| 7-AAD | 500 µl | 1 ml |
| Target | Annexin V |
| Reactivity | General (All species) |
| Conjugation | FITC |
| Excitation/Emission | 499/515 |
| Laser Line | 488 |
| Storage | Store at at 2-8°C in the dark. |
| Validity | 12 months |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |