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Human Apolipoprotein B-100 (APOB100) Protein
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Catalogue No: abx652120
Price:
US$217.50
(Size: 10 µg)
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Human Apolipoprotein B100 (APOB100) Protein is a Recombinant Human protein expressed in E. coli.
Research Articles on Apolipoprotein B-100 (APOB100)
| Target | Apolipoprotein B-100 (APOB100) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 90% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P04114 (UniProt, ExPASy) |
| UniProt Entry Name | APOB_HUMAN |
| Gene Symbol | APOB |
| GeneID | 338 |
| OMIM | 107730 |
| HGNC | 603 |
| KEGG | hsa:338 |
| Ensembl | ENSG00000084674 |
| String | 9606.ENSP00000233242 |
| Molecular Weight | Calculated MW: 21.4 kDa Observed MW (SDS-PAGE): 26 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | His3365-Glu3548 |
| Sequence | HLLSSS SSVIDALQYK LEGTTRLTRK RGLKLATALS LSNKFVEGSH NSTVSLTTKN MEVSVATTTK AQIPILRMNF KQELNGNTKS KPTVSSSMEF KYDFNSSMLY STAKGAVDHK LSLESLTSYF SIESSTKGDV KGSVLSREYS GTIASEANTY LNSKSTRSSV KLQGTSKIDD IWNLEVKE |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Not tested |
| Concentration | Prior to lyophilization: 800 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |