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Beta Actin (ACTB) Antibody
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Catalogue No: abx005571
Price:
US$290.00
(Size: 100 µl)
Orders to United States ship from United States
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Beta actin Antibody is a Mouse Monoclonal antibody against Bet actin. Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta Actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody was generated by full length beta actin protein and can cross-react with other actins.
Research Articles on Beta Actin (ACTB)
| Target | Beta Actin (ACTB) |
| Clonality | Monoclonal |
| Clone | C344 |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF/ICC |
| Host | Mouse |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/5000 - 1/160000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Fusion protein of human β-Actin |
| Isotype | IgG1 Kappa |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P60709 (UniProt, ExPASy) |
| UniProt Entry Name | ACTB_HUMAN |
| GeneID | 60 |
| KEGG | hsa:60 |
| String | 9606.ENSP00000349960 |
| Molecular Weight | Calculated MW: 42 kDa Observed MW: 42 kDa |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
| Concentration | 1.15 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |