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Bad (pS99) Antibody
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Catalogue No: abx031833
Price:
US$326.25
(Size: 80 µl)
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Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X (L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.
Research Articles on Bad (pS99)
| Target | Bad (pS99) |
| Clonality | Polyclonal |
| Target Modification | Ser99 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, IHC, IF/ICC, DB |
| Host | Rabbit |
| Recommended dilutions | IHC-P: 1/50 - 1/100, IF/ICC: 1/200, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q92934 (UniProt, ExPASy) |
| UniProt Secondary AC | O14803, Q6FH21 |
| UniProt Entry Name | BAD_HUMAN |
| NCBI Accession | NP_004313.1 NP_116784.1 |
| KEGG | hsa:572 |
| String | 9606.ENSP00000378040 |
| Molecular Weight | Calculated MW: 18.4 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse and Rat BAD. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |