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Target | Beta Lactamase (LACTB) |
Expression | Recombinant |
Tested Applications | SDS-PAGE |
Host | E. coli |
Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
Form | Lyophilized |
Purity | > 95% (SDS-PAGE) |
Purification | Purified by proprietary chromatographic techniques. |
Reconstitution | Reconstitute in sterile 18 MΩ · cm water to a concentration of at least 100 µg/ml. This stock solution can be further diluted if required. |
Storage | Stable at room temperature for up to 3 weeks. Store below -18 °C for long-term storage. Upon reconstitution, store at 4 °C for up to 7 days and store below -18 °C for long-term storage. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles. |
Stability | Stable at room temperature for up to 3 weeks. |
Molecular Weight | 28.9 kDa |
Sequence | MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW |
Buffer | Prior to lyophilization: 100 mM Tris, pH 7.0. |
Activity | Active |
Biological Activity | One unit will hydrolyze 1.0 µmol of benzylpenicillin per min at pH 7.0 at 25 °C in the presence of EDTA. |
Availability | Shipped within 5-10 working days. |
Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
Directions for use | The enzyme should be used in pH 7.0-8.0 at a temperature not higher than 45 °C. |