Breast Cancer Type 1 Susceptibility Protein Phospho-Ser1423 (BRCA1 pS1423) Antibody

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Catalogue No: abx000284
Price: US$797.50
(Size: 96 tests)

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Western blot analysis of lysates from HT-29 cells, using Phospho-BRCA1-S1423 Antibody at 1/2000 dilution. HT-29 cells were treated by Anisomycin (5 µg/ml) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% BSA. Exposure time: 5s.
Breast Cancer Type 1 Susceptibility Protein Phospho-Ser1423 (BRCA1 pS1423) Antibody Breast Cancer Type 1 Susceptibility Protein Phospho-Ser1423 (BRCA1 pS1423) Antibody Breast Cancer Type 1 Susceptibility Protein Phospho-Ser1423 (BRCA1 pS1423) Antibody Breast Cancer Type 1 Susceptibility Protein Phospho-Ser1423 (BRCA1 pS1423) Antibody Breast Cancer Type 1 Susceptibility Protein Phospho-Ser1423 (BRCA1 pS1423) Antibody

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BRCA1 (pS1423) Antibody is a Rabbit Polyclonal antibody against BRCA1 (pS1423). This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Target Breast Cancer Type 1 Susceptibility Protein Phospho-Ser1423 (BRCA1 pS1423)
Clonality Polyclonal
Target Modification Ser1423
Modification Phosphorylation
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/2000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen A synthetic phosphorylated peptide around S1423 of human BRCA1.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P38398 (UniProt, ExPASy)
UniProt Secondary AC E9PFZ0, O15129, Q1RMC1, Q3LRJ0, Q3LRJ6, Q6IN79, Q7KYU9
UniProt Entry Name BRCA1_HUMAN
Gene Symbol BRCA1
GeneID 672
NCBI Accession NP_009225.1
KEGG hsa:672
String 9606.ENSP00000418960
Molecular Weight Calculated MW: 208 kDa
Observed MW: 270 kDa
Buffer PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Breast Cancer Type 1 Susceptibility Protein Phospho-Ser1423 (BRCA1 pS1423)
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