c-Jun (pS73) Antibody is a Rabbit Polyclonal antibody against c-Jun (pS73). c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).
Target |
c-Jun (pS73) |
Clonality |
Polyclonal |
Reactivity |
Human, Mouse, Rat |
Tested Applications |
WB, IHC |
Host |
Rabbit |
Recommended dilutions |
WB: 1/100 - 1/500, IHC-P: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation |
Unconjugated |
Immunogen |
A synthetic phosphorylated peptide around S73 of human c-Jun. |
Isotype |
IgG |
Form |
Liquid |
Purification |
Purified by affinity chromatography. |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC |
P05412 (UniProt,
ExPASy)
|
UniProt Secondary AC |
Q6FHM7, Q96G93 |
UniProt Entry Name |
JUN_HUMAN |
Gene Symbol |
JUN |
GeneID |
3725
|
NCBI Accession |
NP_002219.1
|
KEGG |
hsa:3725
|
String |
9606.ENSP00000360266
|
Molecular Weight |
Calculated MW: 36 kDa Observed MW: 45-48 kDa |
Buffer |
PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
Concentration |
> 0.2 mg/ml |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |
Research Articles on c-Jun (pS73)