Human Cardiac Troponin T2 (TNNT2) Protein

Human Cardiac Troponin T2 (TNNT2) Protein is a recombinant Human protein expressed in E. coli. The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.

Target	Cardiac Troponin T2 (TNNT2)
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P45379 (UniProt, ExPASy)
UniProt Secondary AC	A2TDB9, A8K3K6, O60214, Q99596, Q99597, Q9BUF6, Q9UM96
UniProt Entry Name	TNNT2_HUMAN
Gene Symbol	TNNT2
GeneID	7139
OMIM	115195
NCBI Accession	NP_001001432.1 NM_001001432.2
HGNC	11949
Ensembl	ENSG00000118194
String	9606.ENSP00000236918
Molecular Weight	Calculated MW: 65.6 kDa Observed MW (SDS-PAGE): 70 kDa
Sequence Fragment	Met1-Lys295
Sequence	MSDIEEVVEE YEEEEQEEAA VEEEEDWRED EDEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK
Tag	N-terminal His and GST tags
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 0.01% Sarcosyl, 5% Trehalose.
Activity	Not tested
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-12 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Cardiac Troponin T2 (TNNT2)

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