Cartilage Staining Solution (Toluidine blue O Method)

Cartilage is a resilient and smooth elastic tissue, rubber-like padding that covers and protects the ends of long bones at the joints and nerves, and is a structural component of the rib cage, the ear, the nose, the bronchial tubes, the intervertebral discs, and many other body components. It is not as hard and rigid as bone, but it is much stiffer and much less flexible than muscle. The matrix of cartilage is made up of glycosaminoglycans, proteoglycans, collagen fibers and, sometimes, elastin.

Toluidine blue, also known as TBO or tolonium chloride (INN) is a blue cationic (basic) dye used in histology and sometimes clinically. Toluidine blue solution is used in testing for lignin, a complex organic molecule that bonds to cellulose fibres and strengthens and hardens the cell walls in plants. A positive toluidine blue test causes the solution to turn from blue to pink.

Target	Cartilage Staining Solution (Toluidine blue O Method)
Form	Liquid
Storage	Store at room temperature.
Buffer	The exact formulation is proprietary.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.
Directions for use	Suggested Protocol: Using conventional methods, decalcify tissue, embed and fix. Submerge the paraffin section into xylene, twice. Wash using a series of increasing alcohol concentration, 1 min each, then wash with water. Dye using the Cartilage Staining Solution. The optimal dyeing time will depend on the tissue and should be determined by the end user. Wash for 2 min using water, then dry by blotting on filter paper. Fix with acetone. Cartilage cells should appear blue-violet. Treat with absolute ethanol and dimethylbenzene, and seal with neutral gum. Cartilaginous, osteogenic cells should appear red-violet; the background is light blue.

Research Articles on Cartilage Staining Solution (Toluidine blue O Method)

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