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Target | CD3 (activation epitope) |
Clonality | Monoclonal |
Clone | Y484 |
Reactivity | Human, Mouse |
Tested Applications | FCM |
Host | Mouse |
Recommended dilutions | FCM: 1-4 µg/ml. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | PE |
Excitation/Emission | 565/575 |
Laser Line | 550 |
Immunogen | Purified Human CD3 proteins isolated from thymus. |
Isotype | IgG1 |
Storage | Store in the dark at 2-8°C. Avoid exposure to light. Do not freeze. |
UniProt Primary AC | P07766 (UniProt, ExPASy) |
Gene Symbol | CD3E |
GeneID | 916 |
KEGG | hsa:916 |
String | 9606.ENSP00000354566 |
Buffer | Stabilizing PBS solution containing 15 mM sodium azide. |
Specificity | The mouse monoclonal antibody recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. |
Concentration | 0.1 mg/ml |
Availability | Shipped within 5-12 working days. |
Note | This product is for research use only. |