M-Phase Inducer Phosphatase 1 Phospho-Ser124 (CDC25A pS124) Antibody

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Target	M-Phase Inducer Phosphatase 1 Phospho-Ser124 (CDC25A pS124)
Clonality	Polyclonal
Target Modification	Ser124
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human CDC25A.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P30304 (UniProt, ExPASy)
UniProt Secondary AC	Q8IZH5, Q96IL3, Q9H2F2
UniProt Entry Name	MPIP1_HUMAN
NCBI Accession	NP_001780.2 NP_963861.1
KEGG	hsa:993
String	9606.ENSP00000303706
Molecular Weight	Calculated MW: 59.1 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Rat CDC25A.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on M-Phase Inducer Phosphatase 1 Phospho-Ser124 (CDC25A pS124)

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