Cell Division Cycle 25C Phospho-Ser216 (CDC25C pS216) Antibody

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Catalogue No: abx123326
Price: US$275.50
(Size: 100 µl)

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Western blot analysis of lysates from HT-29 cells, using Phospho-cdc25C-S216 Antibody at 1/400 dilution. HT-29 cells were treated by λ-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
Cell Division Cycle 25C Phospho-Ser216 (CDC25C pS216) Antibody

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Datasheet SDS
cdc25C (pS216) Antibody is a Rabbit Polyclonal Antibody against cdc25C (pS216).

Target Cell Division Cycle 25C Phospho-Ser216 (CDC25C pS216)
Clonality Polyclonal
Target Modification Ser216
Modification Phosphorylation
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/100 - 1/500, IHC-P: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen A synthetic phosphorylated peptide around S216 of human cdc25C.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P30307 (UniProt, ExPASy)
UniProt Secondary AC D3DQB8, Q96PL3, Q9H168, Q9H2E8, Q9H2E9, Q9H2F1
UniProt Entry Name MPIP3_HUMAN
Gene Symbol CDC25C
GeneID 995
NCBI Accession NP_001781.2
KEGG hsa:995
String 9606.ENSP00000321656
Molecular Weight Calculated MW: 53 kDa
Observed MW: 60 kDa
Buffer PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Cell Division Cycle 25C Phospho-Ser216 (CDC25C pS216)
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