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Target | Cyclin Dependent Kinase 1 Phospho-Thr14 (CDK1 pT14) |
Clonality | Polyclonal |
Target Modification | Thr14 |
Modification | Phosphorylation |
Reactivity | Human, Mouse, Rat |
Tested Applications | ELISA, WB, IF/ICC |
Host | Rabbit |
Recommended dilutions | ELISA: 1/1000, WB: 1/500 - 1/3000, IF/ICC: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human CDK1/CDC2 around the phosphorylation site of threonine 14 (E-G-TP-Y-G). |
Isotype | IgG |
Form | Liquid |
Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P06493 (UniProt, ExPASy) P24941 (UniProt, ExPASy) Q00526 (UniProt, ExPASy) |
Enzyme Commission Number | EC 2.7.11.22 EC 2.7.11.23 |
Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
Specificity | Detects endogenous levels of CDK1/CDC2 only when phosphorylated at threonine 14. |
Concentration | 1 mg/ml |
Availability | Shipped within 5-10 working days. |
Note | This product is for research use only. |