Collagen Type II Alpha 1 (COL2A1) Antibody

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Catalogue No: abx001311
Price: US$217.50
(Size: 20 µl)
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Western blot analysis of lysates from Mouse skeletal muscle, using COL2A1 Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.
Collagen Type II Alpha 1 (COL2A1) Antibody Collagen Type II Alpha 1 (COL2A1) Antibody

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Datasheet SDS
COL2A1 Antibody is a Rabbit Polyclonal antibody against COL2A1. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.

Target Collagen Type II Alpha 1 (COL2A1)
Clonality Polyclonal
Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IHC-P: 1/1000 - 1/5000, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1220-1282 of human COL2A1.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P02458 (UniProt, ExPASy)
Gene Symbol COL2A1
GeneID 1280
NCBI Accession NP_001835.3
Molecular Weight Calculated MW: 142 kDa
Observed MW: 141 kDa
Buffer PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Collagen Type II Alpha 1 (COL2A1)


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