Complement Fragment C3d (C3d) Antibody

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Catalogue No: abx227113
Price: US$290.00
(Size: 40 µl)
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IHC-P analysis of skin biopsy from the lesion of the early pemphigus vulgaris (without blister formation), showing strong positive intraepidermal intercellular immunostaining (4 µm section).
Complement Fragment C3d (C3d) Antibody

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Datasheet SDS
Complement Fragment C3d (C3d) Antibody is a Rabbit Monoclonal antibody for the detection of C3d complement.

Target Complement Fragment C3d (C3d)
Clonality Monoclonal
Clone I808
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the N-terminal region of human C3d complement fragment.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC P01024 (UniProt, ExPASy)
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use Suggested IHC-P Protocol
  1. Preparation of Tris-EDTA Buffer (10 mM Tris Base, 1 mM EDTA solution, 0.05% Tween-20, pH 9.0): Dissolve 1.21 g Tris and 0.37 g EDTA in 700 ml of distilled water and mix. Adjust pH to 9.0 with 1 M HCl and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  2. Preparation of Wash Buffer: Use 0.05 M Tris-HCl, pH 7.6, containing 0.2% Tween-20; or PBS containing 0.2% Tween-20.
  3. Preparation of Copper Sulfate Solution: Dissolve 0.90 g NaCl and 0.50 g CuSO4.5H2O in 100 ml distilled water.
  4. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
  5. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  6. Rinse twice in distilled water, 5 minutes each.
  7. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  8. Rinse twice in distilled water, 5 minutes each.
  9. Antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, and incubate in a water bath for 20-25 minutes at 96-98 °C.
  10. Remove the slide from the water bath and allow to stand at room temperature (in Tris-EDTA buffer, pH 9.0) for 20 minutes.
  11. Rinse twice in distilled water, 5 minutes each.
  12. Wash twice in Wash Buffer, 5 minutes each.
  13. Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (≥ 1 mg/ml) to dilute this antibody.
  14. Rinse three times in distilled water, 5 minutes each.
  15. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  16. Wash three times in Wash Buffer, 5 minutes each.
  17. Apply the DAB chromagen for 1-3 minutes.
  18. Wash twice in distilled water, 5 minutes each.
  19. Rinse in Copper Sulfate Solution.
  20. Wash in distilled water for 2 minutes.
  21. Stain in hematoxylin for 5 minutes.
  22. Wash three times in distilled water, 2 minutes each.
  23. Rinse in 37 mM ammonium hydroxide solution for 1 minute.
  24. Wash in distilled water for 2 minutes.
  25. Mount the slide for observation.
Research Articles on Complement Fragment C3d (C3d)


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