Carnitine Palmitoyltransferase 1A, Liver (CPT1A) Antibody

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Catalogue No: abx004057
Price: US$797.50
(Size: 96 tests)

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Western blot analysis of lysates from HeLa cells using CPT1A Antibody at 1/2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
Carnitine Palmitoyltransferase 1A, Liver (CPT1A) Antibody Carnitine Palmitoyltransferase 1A, Liver (CPT1A) Antibody Carnitine Palmitoyltransferase 1A, Liver (CPT1A) Antibody

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Datasheet SDS
CPT1A Antibody is a Rabbit Polyclonal antibody against CPT1A. The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Target Carnitine Palmitoyltransferase 1A, Liver (CPT1A)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/1000 - 1/5000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P50416 (UniProt, ExPASy)
UniProt Secondary AC Q8TCU0, Q9BWK0
UniProt Entry Name CPT1A_HUMAN
Gene Symbol CPT1A
GeneID 1374
NCBI Accession NP_001867.2
KEGG hsa:1374
String 9606.ENSP00000265641
Molecular Weight Calculated MW: 88 kDa
Observed MW: 88 kDa
Buffer PBS, pH 7.3, containing 0.09% sodium azide, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Carnitine Palmitoyltransferase 1A, Liver (CPT1A)
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