Creatinine Assay Kit can be used to detect Creatinine in serum, plasma and urine using a microplate reader. Creatinine (Cr) can be catalyzed by creatinase, generating creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase to form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3-hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase, forming a pink compound. The Creatinine concentration can be calculated by measuring the absorbance at 515 nm.

Target	Creatinine
Reactivity	General (All species)
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity	The validity for this kit is 12 months.
Buffer	Not applicable.
Test Range	20.45 µmol/L – 800 µmol/L
Sensitivity	3.8 µmol/L
Detection Method	Colorimetric
Assay Data	Quantitative
Sample Type	Serum, plasma and urine.
CAS Number	60-27-5
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Creatinine

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