Alpha-Crystallin A Chain (CRYAA) Antibody

CRYAA Antibody is a Rabbit Polyclonal antibody against CRYAA. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).

Target	Alpha-Crystallin A Chain (CRYAA)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat, Zebrafish
Tested Applications	WB, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/2000 - 1/6000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human CRYAA.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P02489 (UniProt, ExPASy)
UniProt Secondary AC	Q53X53
UniProt Entry Name	CRYAA_HUMAN
Gene Symbol	CRYAA
GeneID	1409
NCBI Accession	NP_000385.1
KEGG	hsa:102724652 hsa:1409
String	9606.ENSP00000482816
Molecular Weight	Calculated MW: 20 kDa Observed MW: 19 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Alpha-Crystallin A Chain (CRYAA)

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