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Human Cytochrome P450 1A2 (CYP1A2) Protein (Active)
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Catalogue No: abx651319
Price:
US$855.50
(Size: 50 µg)
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Cytochrome P450 1A2 (CYP1A2) Protein (Active) is an active protein from Human.
Research Articles on Cytochrome P450 1A2 (CYP1A2)
| Target | Cytochrome P450 1A2 (CYP1A2) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 95% |
| Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P05177 (UniProt, ExPASy) |
| UniProt Secondary AC | Q16754, Q6NWU3, Q6NWU5, Q9BXX7, Q9UK49 |
| UniProt Entry Name | CP1A2_HUMAN |
| KEGG | hsa:1544 |
| String | 9606.ENSP00000342007 |
| Molecular Weight | Calculated MW: 55.2 kDa Observed MW: 55 kDa |
| Sequence Fragment | Ala2-Ser231 |
| Sequence | ALSQSVPFS ATELLLASAI FCLVFWVLKG LRPRVPKGLK SPPEPWGWPL LGHVLTLGKN PHLALSRMSQ RYGDVLQIRI GSTPVLVLSR LDTIRQALVR QGDDFKGRPD LYTSTLITDG QSLTFSTDSG PVWAARRRLA QNALNTFSIA SDPASSSSCY LEEHVSKEAK ALISRLQELM AGPGHFDPYN QVVVSVANVI GAMCFGQHFP ESSDEMLSLV KNTHEFVETA S |
| Tag | N-terminal His tag and GST tag |
| Buffer | Prior to lyophilization: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | Cytochrome P450 1A2 (CYP1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acid Ceramidase (ASAH1) has been identified as an interactor of CYP1A2 through affinity capture-MS, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ASAH1-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-CYP1A2 monoclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of CYP1A2 and ASAH1 is shown in Figure 4. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |