Human Cytochrome P450 1A2 (CYP1A2) Protein (Active)

Cytochrome P450 1A2 (CYP1A2) Protein (Active) is an active protein from Human.

Target	Cytochrome P450 1A2 (CYP1A2)
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	Reconstitute in ddH₂O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P05177 (UniProt, ExPASy)
UniProt Secondary AC	Q16754, Q6NWU3, Q6NWU5, Q9BXX7, Q9UK49
UniProt Entry Name	CP1A2_HUMAN
KEGG	hsa:1544
String	9606.ENSP00000342007
Molecular Weight	Calculated MW: 55.2 kDa Observed MW: 55 kDa
Sequence Fragment	Ala2-Ser231
Sequence	ALSQSVPFS ATELLLASAI FCLVFWVLKG LRPRVPKGLK SPPEPWGWPL LGHVLTLGKN PHLALSRMSQ RYGDVLQIRI GSTPVLVLSR LDTIRQALVR QGDDFKGRPD LYTSTLITDG QSLTFSTDSG PVWAARRRLA QNALNTFSIA SDPASSSSCY LEEHVSKEAK ALISRLQELM AGPGHFDPYN QVVVSVANVI GAMCFGQHFP ESSDEMLSLV KNTHEFVETA S
Tag	N-terminal His tag and GST tag
Buffer	Prior to lyophilization: 100 mM NaHCO₃, 500 mM NaCl, pH 8.3, containing 0.01% Sarcosyl, 5% Trehalose.
Activity	Active
Biological Activity	Cytochrome P450 1A2 (CYP1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acid Ceramidase (ASAH1) has been identified as an interactor of CYP1A2 through affinity capture-MS, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ASAH1-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-CYP1A2 monoclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of CYP1A2 and ASAH1 is shown in Figure 4.
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Cytochrome P450 1A2 (CYP1A2)

Human Cytochrome P450 1A2 (CYP1A2) Protein (Active)

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