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DDDDK-Tag Antibody (Magnetic Beads)
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Catalogue No: abx005594
Price:
US$275.50
(Size: 100 µl)
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DDDDK-Tag Antibody (Magnetic Beads) is a Mouse Monoclonal antibody conjugated to Magnetic Beads against DDDDK-Tag. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.
Research Articles on DDDDK-Tag
| Target | DDDDK-Tag |
| Clonality | Monoclonal |
| Reactivity | General (All species) |
| Tested Applications | IP |
| Host | Mouse |
| Recommended dilutions | IP: 30 µl antibody (bead slurry) per 200 µg - 400 µg extracts of whole cells. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide corresponding to DDDDK tag. |
| Isotype | IgG1 |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at 2°C to 8°C upon receipt. |
| Molecular Weight | Observed MW: 50/48 kDa |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |