DDDDK-Tag Antibody
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Price:
US$333.50
(Size: 100 µl)
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DDDDK-Tag Antibody is a Mouse Monoclonal antibody against DDDDK-Tag. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.
Target |
DDDDK-Tag |
Clonality |
Monoclonal |
Reactivity |
General (All species) |
Tested Applications |
WB, IF/ICC, IP |
Host |
Mouse |
Recommended dilutions |
WB: 1/1000 - 1/6000, IF/ICC: 1/50 - 1/200, IP: 0.5-4 µg/200-400 µg whole cell extracts. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation |
Unconjugated |
Immunogen |
A synthetic peptide corresponding to DDDDK tag. |
Isotype |
IgG1 Kappa |
Form |
Liquid |
Purification |
Purified by affinity chromatography. |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Molecular Weight |
Observed MW: 55/48/40 kDa |
Buffer |
PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
Concentration |
> 0.2 mg/ml |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |
Research Articles on DDDDK-Tag