Dihydrolipoyl Transacetylase (DLAT) Antibody

DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.

Target	Dihydrolipoyl Transacetylase (DLAT)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC, FCM
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/50, FCM: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 579-607 amino acids from the C-terminal region of human DLAT.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P10515 (UniProt, ExPASy)
UniProt Secondary AC	Q16783, Q53EP3
UniProt Entry Name	ODP2_HUMAN
Gene Symbol	DLAT
KEGG	hsa:1737
String	9606.ENSP00000280346
Molecular Weight	Calculated MW: 69 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Dihydrolipoyl Transacetylase (DLAT)

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