DMEM, High Glucose (Powder)

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting mammalian cell growth. It contains a high concentration of glucose along with various amino acids, vitamins and inorganic salts essential for cell growth, though does not contain proteins, lipids or growth factors.

The 1 × 10 L size is provided as 1 bottle containing lyophilized powder for 10 L of 1X DMEM. The 1 × 50 L size is provided as 1 bottle containing lyophilized powder for 50 L of 1X DMEM.

Target	DMEM
Form	Lyophilized
Reconstitution	For preparation of 1 L of DMEM, High Glucose: Dissolve 13.48 g or 1 pouch of DMEM, High Glucose (Powder) in ultrapure water. Add 2.2 g of NaHCO3 and add ultrapure water until it is fully dissolved. If required, adjust the pH with 1 M NaOH or 1 M HCl. Add ultrapure water to a final volume of 1 L. For cell culture applications, sterilize the solution using a 0.22 µm filter under aseptic conditions.
Storage	Store between 2-8 °C. Avoid exposure to light.
Validity	Up to 12 months.
Buffer	After reconstitution: pH 7.2-7.4, containing 4500 mg/L L-glucose, 4 mM L-glutamine, 15 mg/L phenol red and 1 mM sodium pyruvate. Does not contain HEPES or NaHCO3.
Concentration	1X
Availability	Shipped within 5-7 working days.
Note	This product is for research use only.
Directions for use	This product should be used with serum (or equivalent) supplementation. Use aseptic techniques to avoid contamination.

Research Articles on DMEM

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