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Type-2 Restriction Enzyme DpnI (DMT) Enzyme
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Catalogue No: abx098137
Price:
US$754.00
(Size: 200 U)
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Datasheet SDS
DMT cuts the sequence GAmTC (where A is methylated) but does not cut the sequence GATC (where A is not methylated). This enzyme can cut DNA prepared from the most commonly used E. coli strains (dam+ strain), but does not cut PCR products. This product is suitable for use in in vitro site-directed DNA mutagenesis, and degradation of methylated plasmid templates. Restriction site:
CH3
|↓
5' GA|TC 3'
3' CT|AG 5'
↑|
CH3
Research Articles on Type-2 Restriction Enzyme DpnI (DMT)
CH3
|↓
5' GA|TC 3'
3' CT|AG 5'
↑|
CH3
| Target | Type-2 Restriction Enzyme DpnI (DMT) |
| Storage | Store at -20°C. Stable for 12 months from date of receipt. |
| Biological Activity | One unit (U) is defined as the amount of enzyme required for completely digesting 1 µg methylated pBR322 DNA at 37 °C (reaction time: 1 h, reaction volume: 50 µl). |
| Availability | Shipped within 10-20 working days. |
| Note | This product is for research use only. This product is shipped with dry ice. |