DNA Mismatch Repair Protein MutS (MutS) Protein

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Catalogue No: abx680413
Price: US$261.00
(Size: 10 µg)

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DNA Mismatch Repair Protein MutS (MutS) Protein

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Datasheet SDS
DNA Mismatch Repair Protein MutS (MutS) Protein is a recombinant protein produced in a prokaryotic expression system (E. coli), consisting of a single, non-glycosylated polypeptide chain containing 829 amino acids. MutS protein is fused to a His tag at the C-terminus.

Target DNA Mismatch Repair Protein MutS (MutS)
Origin Bacteria
Expression Recombinant
Tested Applications SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Liquid
Purity > 95% (SDS-PAGE)
Purification Purified by proprietary chromatographic techniques. Sterile filtered.
Storage Store at 4 °C if the entire vial will be used within 4 weeks. Store at -20 °C for long-term storage. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.
Molecular Weight 92.8 kDa
Sequence Fragment 829 AA
Sequence MEGMLKGEGP GPLPPLLQQY VELRDQYPDY LLLFQVGDFY ECFGEDAERL ARALGLVLTH KTSKDFTTPM AGIPLRAFEA YAERLLKMGF RLAVADQVEP AEEAEGLVRR EVTQLLTPGT LLQESLLPRE ANYLAAIATG DGWGLAFLDV STGEFKGTVL KSKSALYDEL FRHRPAEVLL APELLENGAF LDEFRKRFPV MLSEAPFEPE GEGPLALRRA RGALLAYAQR TQGGALSLQP FRFYDPGAFM RLPEATLRAL EVFEPLRGQD TLFSVLDETR TAPGRRLLQS WLRHPLLDRG PLEARLDRVE GFVREGALRE GVRRLLYRLA DLERLATRLE LGRASPKDLG ALRRSLQILP ELRALLGEEV GLPDLSPLKE ELEAALVEDP PLKVSEGGLI REGYDPDLDA LRAAHREGVA YFLELEERER ERTGIPTLKV GYNAVFGYYL EVTRPYYERV PKEYRPVQTL KDRQRYTLPE MKEKEREVYR LEALIRRREE EVFLEVRERA KRQAEALREA ARILAELDVY AALAEVAVRY GYVRPRFGDR LQIRAGRHPV VERRTEFVPN DLEMAHELVL ITGPNMAGKS TFLRQTALIA LLAQVGSFVP AEEAHLPLFD GIYTRIGASD DLAGGKSTFM VEMEEVALIL KEATENSLVL LDEVGRGTSS LDGVAIATAV AEALHERRAY TLFATHYFEL TALGLPRLKN LHVAAREEAG GLVFYHQVLP GPASKSYGVE VAAMAGLPKE VVARARALLQ AMAARREGAL DAVLERLLAL DPDRLTPLEA LRLLQELKAL ALGAPLDTMK GLEHHHHHH
Tag C-terminal His tag
Buffer Storage buffer: 20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 0.1 mM EDTA, 1 mM DTT and 50% Glycerol.
Reaction buffer: 100 mM KCl, 50 mM Tris-HCl, pH 8.5, 20 mM MgCl2, 0.1 mM EDTA, 1 mM DTT and 2% Glycerol, 65°C.
Concentration 1.4 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Recommended PCR Procedure:

  1. After first round PCR, purify PCR fragments using a PCR purification kit, elution in dH2O.
  2. Dilute PCR product to 250 ng/µl in 10 mM Tris–HCl, pH 7.8, 50 mM NaCl and heat to 95 °C for 5 min followed by cooling at 0.1 °C/s to 25 °C.
  3. Add binding buffer (20 mM Tris–HCl, pH 7.8, 10 mM NaCl, 5 mM MgCl 2 , 1 mM DTT and 5% glycerol) to annealed PCR product, adjust DNA concentration to 11.5 ng/µl, add MutS dimers to 950 nM.
  4. Incubate the mixture at room temperature for 10 min, then add an equal volume of Ni-NTA beads pre-equilibrated with 1X binding buffer, and incubate for 30 min at room temperature.
  5. Genlty spin down beads and retain supernatant for further processing.
Research Articles on DNA Mismatch Repair Protein MutS (MutS)
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