DNA Replication ATP-Dependent Helicase/Nuclease DNA2 (DNA2) Antibody

DNA Replication ATP-Dependent Helicase/Nuclease DNA2 (DNA2) Antibody is a Rabbit Polyclonal antibody for the detection of DNA2.

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA) -dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Target	DNA Replication ATP-Dependent Helicase/Nuclease DNA2 (DNA2)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IHC: 1/50 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	DNA replication helicase 2 homolog
Isotype	IgG
Form	Liquid
Purity	≥ 95% (SDS-PAGE)
Purification	Purified by immunogen affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Validity	12 months.
UniProt Primary AC	P51530 (UniProt, ExPASy)
UniProt Secondary AC	Q2NKM1, Q5TC49, Q5TC50, Q6P455, Q6PI80, Q7Z6H9, Q8N346
UniProt Entry Name	DNA2_HUMAN
Gene Symbol	DNA2
GeneID	1763
OMIM	601810
NCBI Accession	NP_001073918.2 NM_001080449.2
KEGG	hsa:1763
String	9606.ENSP00000351185
Molecular Weight	Observed MW: 127 kDa
Buffer	PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Concentration	2 mg/ml
Availability	Shipped within 5-12 working days.
Note	This product is for research use only.

Research Articles on DNA Replication ATP-Dependent Helicase/Nuclease DNA2 (DNA2)

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