Dnmt1 (pS714) Antibody

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

Target	Dnmt1 (pS714)
Clonality	Polyclonal
Target Modification	Ser714
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, DB
Host	Rabbit
Recommended dilutions	DB: 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S714 of human Dnmt1.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P26358 (UniProt, ExPASy)
UniProt Secondary AC	A0AV63, B7ZLW6, Q9UHG5, Q9ULA2, Q9UMZ6
UniProt Entry Name	DNMT1_HUMAN
KEGG	hsa:1786
String	9606.ENSP00000352516
Molecular Weight	Calculated MW: 183 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Cow DNMT1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Dnmt1 (pS714)

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