Ebola Virus Mucin-Domain-Deleted Glycoprotein (EBOV GPdMuc) Antibody

Ebola Virus Mucin-Domain-Deleted Glycoprotein (EBOV GPdMuc) Antibody (Subtype Sudan, Strain Gulu) is a Rabbit Monoclonal antibody for the detection of Ebola Virus Mucin-Domain-Deleted Glycoprotein (EBOV GPdMuc).

The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD); the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry; the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.

Target	Ebola Virus Mucin-Domain-Deleted Glycoprotein (EBOV GPdMuc)
Clonality	Monoclonal
Reactivity	Virus
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	ELISA: 1/5000 - 1/10000, WB: 1/1000 - 1/5000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP1 (mucin domain deleted) Protein
Isotype	IgG
Form	Liquid
Purification	Purified by Protein A affinity chromatography. 0.2 µm filtered.
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Buffer	PBS.
Concentration	1 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.
Directions for use	Centrifuge before opening to ensure complete recovery of vial contents.

Research Articles on Ebola Virus Mucin-Domain-Deleted Glycoprotein (EBOV GPdMuc)

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