Non-Liposomal Transfection Reagent

REQUEST MORE INFO
Catalogue No: abx098880
Price: US$667.00
(Size: 0.75 ml)

Orders to United States ship from United States

Reviews: 1 Publications and Reviews
(1 Publications and Reviews)
See Related Products
Non-Liposomal Transfection Reagent

Click on the image to see the image legend

Available Options

* Size:


Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
Non-Liposomal Transfection Reagent contains a non-liposomal formulation designed to transfect DNA and RNA into a wide variety of eukaryotic cell lines with high efficiency and low toxicity. Primary cells and other difficult-to-transfect cells can also be effectively transfected using this reagent. This reagent can be used in the presence of serum and antibiotics.

Recommended volumes of culture medium, nucleic acid and Non-Liposomal Transfection Reagent for transfection in different cell culture plates:

Culture Vessel Surface Area per well (cm2) Plating Medium Volume (ml) Dilution Volume (µl) DNA Transfection siRNA Transfection
DNA (µg) Non-Liposomal Transfection Reagent (µl) siRNA (pmol) Non-Liposomal Transfection Reagent (µl)
96-well plate 0.3 0.1 10 0.2 0.2 - 0.6 10 0.25 - 0.65
48-well plate 1 0.25 25 0.4 0.4 - 1.2 20 0.5 - 1.25
24-well plate 2 0.5 50 0.8 0.8 - 2.4 50 1 - 2.5
12-well plate
 4 1 100 1.6 1.6 - 4.8 100 2 - 2.5
6-well plate 10 2 200 4 4 - 12 250 5 - 12.5
35 mm 10 2 200 4 4 - 12 250 5 - 12.5
60 mm 20 5 500 8 8 - 24 500 10 - 25
10 cm 60 10 1000 24 24 - 72 1500 30 - 75
T 25 25 6 500 10 10 - 30 625 12.5 - 31.5
T 75 75 20 1000 30 30 - 90 2000 40 - 100


Target Non-Liposomal Transfection Reagent
Form Liquid
Storage Store between 2-8 °C. Stable for 12 months from date of receipt.
Sample Type Tested cell types: A549, B16-F10, BHK-21, BTC, CEF, CHO, COS-1, DLD-1, HCT-116, HEK-293, HEK-293T, HeLa, HepG2, HL-60, K562, L929, MARC-145, MCF-7, MEF, MIA PaCa-2, Neuro-2a, NIH/3T3, NRK, P815, PANC-1, PT67, SGC-7901, SH-SY5Y, STO, Vero.
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Plasmid DNA Transfection: (for a 24-well plate)

  1. Seed cells: plate 2-6 × 104 cells in each well so that there is 70-90% confluence at time of transfection.
  2. Incubate for 12-24 hours.
  3. Dilute 0.8 µg of plasmid with 50 µl of reduced serum Minimal Essential Medium (MEM).
  4. Add 1.6 µl of premixed Non-Liposomal Transfection Reagent and mix gently.
  5. Allow to stand for 15-20 minutes at room temperature.
  6. Add the solution to each well containing cells and medium. Incubate cells at 37 °C in a CO2 incubator.
  7. Change medium 4-6 hours post-transfection (optional). Incubate cells for 18-72 hours.

siRNA Transfection: (for a 24-well plate)

  1. Seed cells so that there is 50-70% confluence at time of transfection.
  2. Incubate for 12-24 hours.
  3. Dilute 50 pmol of siRNA with 50 µl of reduced serum Minimal Essential Medium (MEM).
  4. Add 2 µl of premixed Non-Liposomal Transfection Reagent and mix gently.
  5. Allow to stand for 15-20 minutes at room temperature.
  6. Add the solution to each well containing cells and medium. Incubate cells at 37 °C in a CO2 incubator.
  7. Change medium 4-6 hours post-transfection (optional). Incubate cells for 18-72 hours.
Research Articles on Non-Liposomal Transfection Reagent


Write a review

Continue

Related Products

Tags:
(Click to show)