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EP300 (pS89) Antibody
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Catalogue No: abx031894
Price:
US$326.25
(Size: 80 µl)
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EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
Research Articles on EP300 (pS89)
| Target | EP300 (pS89) |
| Clonality | Polyclonal |
| Target Modification | Ser89 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, IHC, DB |
| Host | Rabbit |
| Recommended dilutions | IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q09472 (UniProt, ExPASy) |
| UniProt Secondary AC | B1AKC2 |
| UniProt Entry Name | EP300_HUMAN |
| NCBI Accession | NP_001420.2 |
| KEGG | hsa:2033 |
| String | 9606.ENSP00000263253 |
| Molecular Weight | Calculated MW: 264 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse EP300. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |