Epidermal Growth Factor Receptor Substrate 15-Like 1 (EPS15L1) Antibody

Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

Target	Epidermal Growth Factor Receptor Substrate 15-Like 1 (EPS15L1)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 186-216 amino acids from the N-terminal region of human EPS15R.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9UBC2 (UniProt, ExPASy)
UniProt Secondary AC	A2RRF3, A5PL29, B4DKA3
UniProt Entry Name	EP15R_HUMAN
String	9606.ENSP00000393313
Molecular Weight	Calculated MW: 94.3 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Epidermal Growth Factor Receptor Substrate 15-Like 1 (EPS15L1)

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