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Eukaryotic Translation Initiation Factor 2 Alpha Kinase 1 (EIF2AK1) Antibody
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Catalogue No: abx149959
Price:
US$406.00
(Size: 100 µg)
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Eukaryotic Translation Initiation Factor 2 Alpha Kinase 1 (EIF2AK1) Antibody is a Rabbit Polyclonal antibody for the detection of EIF2AK1. Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity).
Research Articles on Eukaryotic Translation Initiation Factor 2 Alpha Kinase 1 (EIF2AK1)
| Target | Eukaryotic Translation Initiation Factor 2 Alpha Kinase 1 (EIF2AK1) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/500 - 1/2000, IHC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | eukaryotic translation initiation factor 2-alpha kinase 1 |
| Isotype | IgG |
| Form | Liquid |
| Purity | ≥ 95% (SDS-PAGE) |
| Purification | Purified by immunogen affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Validity | 12 months. |
| UniProt Primary AC | Q9BQI3 (UniProt, ExPASy) |
| UniProt Entry Name | E2AK1_HUMAN |
| Gene Symbol | EIF2AK1 |
| GeneID | 27102 |
| OMIM | 613635 |
| HGNC | 24921 |
| Ensembl | ENSG00000086232 |
| String | 9606.ENSP00000199389 |
| Enzyme Commission Number | EC 2.7.11.1 |
| Molecular Weight | Observed MW: 60-71 kDa |
| Buffer | PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol. |
| Concentration | 2 mg/ml |
| Availability | Shipped within 5-12 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |