Abbexa

High EEO agarose is an agarose designed for the analysis of molecules that exhibit high migration to the cathode. Suitable for the analysis of different nucleic acid fragmnents, in separation techniques such as electrophoresis counterimmunoelectrophoresis, chromotography, blotting and others used in the field of biochemestry and molecular biology.

Specification:

Moisture	≤ 10%
Gel strength (1.5% gel)	≥ 1200 g/cm²
Gelling point (1.5% gel)	41-45 °C
Melting point (1.5% gel)	84-88 °C

Target	Agarose High EEO
Storage	Store at room temperature.
Availability	Shipped within 10-15 working days.
Note	This product is for research use only.
Directions for use	Preparation of agarose gel in a microwave: Determine the amount of gel and reagents required to prepare the gel at the desired concentration. Add an appropriate volume of run buffer (TAE, TBE to 1X). Homogenize and microwave for 2 - 4 minutes, in cycles of 30 seconds, until the sample is completely dissolved. It is recommended to run the microwave at medium power. Caution: Use necessary protection for the handling of hot material. Add staining reagent (e.g. Ethidium Bromide) and homogenize. Allow to cool to 60° C before casting. After casting, allow to cool and gel to room temperature Mount the electrophoresis chamber, load samples and run buffer (same buffer with which the gel was prepared) and start the electrophoretic run. Preparation agarose gel on a hot plate. Determine the amount of gel and reagents required to prepare the gel at the desired concentration. Add an appropriate volume of run buffer (TAE, TBE to 1X). Homogenize and heat on a hot plate with stirring. Once boiling has started, start a timer for 20 minutes (use a lid or refrigerant system to avoid concentrating the sample). Add staining reagent (e.g. Ethidium Bromide) and homogenize. Allow to cool to 60° C before casting. After casting, allow to cool and gel to room temperature Mount the electrophoresis chamber, load samples and run buffer (same buffer with which the gel was prepared) and start the electrophoretic run.

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Agarose High EEO

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