FastPfu DNA Polymerase Enzyme (with 2.5 mM dNTPs)

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Catalogue No: abx071019
Price: US$362.50
(Size: 250 U)
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FastPfu DNA Polymerase Enzyme (with 2.5 mM dNTPs)

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Datasheet SDS
FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase with an extension rate of 4 kb/min. Amplification of genomic DNA fragment up to 15 kb. Amplification of plasmid DNA fragment up to 20 kb.

Contents:

Component 250 U 500 U 3 kU
FastPfu DNA Polymerase 1 × 250 U 1 × 500 U6 × 500 U
5X FastPfu Buffer 1.2 ml 2 × 1.2 ml 12 × 1.2 ml
2.5 mM dNTPs500 µl 1 ml 6 × 1 ml
50 mM MgSO4 200 µl 400 µl 1 ml
PCR Stimulant 200 µl 400 µl 1 ml
6X DNA Loading Buffer 500 µl 1 ml 2 × 1 ml


Target FastPfu DNA Polymerase
Tested Applications PCR
Conjugation Unconjugated
Storage Store at -20°C for up to 2 years. Avoid repeated freeze/thaw cycles.
Buffer FastPfu DNA Polymerase: Contains 50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol.
FastPfu Buffer: Contains 100 mM Tris-SO4 (pH 9.2), 200 mM KCl, 10 mM MgSO4, 10% glycerol.
Concentration 2.5 U/µl
Availability Shipped within 10-20 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Directions for use Reaction Components:

Component Volume Final Concentration
Template Variable as required
Forward Primer (10 µM) 1 µl 0.2 µM
Reverse Primer (10 µM) 1 µl 0.2 µM
5X FastPfu Buffer 10 µl 1X
2.5 mM dNTPs 4 µl 0.2 mM
FastPfu DNA Polymerase 1 µl 2.5 U
Nuclease-Free ddH2O Variable N/A
Total Volume 50 µl N/A

Suggested Reaction Conditions:

Template Quantity
Genomic DNA10-100 ng Genomic DNA
Plasmid DNA 1-30 ng Plasmid DNA
cDNA 1-2 µl cDNA from RT reaction
(50-500 ng RNA for RT reaction)

Thermal Cycling Conditions:

Number of Cycles Temperature Time
1 cycle 95 °C 2 min
30-35 cycles 95 °C 20 seconds
TM - 5 °C 20 seconds
72 °C 6 kb/min
1 cycle 72 °C5 min

Notes:
  • The PCR Stimulant can be used to optimize the amplification of complex templates or high GC/AT templates. The recommended working concentration is 0.5X - 2X (concentration of the provided stock solution is 5X).
  • It is recommended to add the FastPfu DNA Polymerase last in the reaction system.
  • For GC-rich templates, the recommended denaturation temperature is 98 °C.
  • If precipitates in the FastPfu Buffer are observed, warm to 37 °C in a water bath and mix before use.
Research Articles on FastPfu DNA Polymerase


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