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Target | FGFR (pY653/654) |
Clonality | Polyclonal |
Target Modification | Tyr653/654 |
Modification | Phosphorylation |
Reactivity | Human, Mouse |
Tested Applications | ELISA, WB, IHC |
Host | Rabbit |
Recommended dilutions | WB: 1/1000, IHC-P: 1/100 - 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y653/654 of human FGFR. |
Isotype | IgG |
Form | Liquid |
Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P11362 (UniProt, ExPASy) |
UniProt Entry Name | FGFR1_HUMAN |
GeneID | 2260 |
NCBI Accession | NP_001167534.1 NP_001167535.1 NP_001167536.1 NP_001167537.1 NP_001167538.1 NP_056934.2 NP_075593.1 NP_075594.1 NP_075598.2 |
KEGG | hsa:2260 |
String | 9606.ENSP00000393312 |
Molecular Weight | Calculated MW: 91.9 kDa |
Buffer | PBS containing 0.09% sodium azide. |
Specificity | Predicted to react with Rat, Chicken, Xenopus and Zebrafish FGFR1. |
Availability | Please enquire. |
Note | This product is for research use only. |