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Target | Fibrinogen (FG) |
Origin | Human |
Expression | Native |
Tested Applications | WB, SDS-PAGE |
Host | Human |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 92% (SDS-PAGE) |
Purification | Purified by salt co-precipitation and ion-exchange chromatography. |
Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P02671 (UniProt, ExPASy) P02675 (UniProt, ExPASy) P02679 (UniProt, ExPASy) |
Molecular Weight | Calculated MW: 340 kDa Observed MW: 70 kDa, 56 kDa, 52 kDa. Possible reasons why the actual band size differs from the predicted band size: Fibrinogen is an abundant plasma protein (intact MW: 340 kDa), composed of 3 pairs of polypeptide chains joined by disulfide bonds: alpha (70 kDa), beta (56 kDa) and gamma (52 kDa). |
Sequence | This product is a native protein and the sequence has not been determined. |
Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
Activity | Not tested |
Concentration | Prior to lyophilization: 600 µg/ml |
Availability | Shipped within 5-7 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |