Rat Fibroblast Growth Factor 21 (FGF21) Protein

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Catalogue No: abx167639
Price: US$261.00
(Size: 10 µg)

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SDS-PAGE analysis of recombinant Fibroblast Growth Factor 21 Protein.
Rat Fibroblast Growth Factor 21 (FGF21) Protein

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Datasheet SDS
Fibroblast Growth Factor 21 Protein is a recombinant Rat protein expressed in E. coli.

Target Fibroblast Growth Factor 21 (FGF21)
Origin Rat
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 90%
Reconstitution Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q8VI80 (UniProt, ExPASy)
UniProt Entry Name Q8VI80_RAT
Gene Symbol FGF21
GeneID 170580
Ensembl ENSRNOG00000020990
String 10116.ENSRNOP00000028490
Molecular Weight Calculated MW: 23.9 kDa
Observed MW (SDS-PAGE): 27 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Gly25-Ser208
Sequence GVCEAY PISDSSPLLQ FGGQVRQRYL YTDDDQDTEA HLEIREDGTV VGTAHRSPES LLELKALKPG VIQILGVKAS RFLCQQPDGT LYGSPHFDPE ACSFRELLLK DGYNVYQSEA HGLPLRLPQK DSQDPATRGP VRFLPMPGLP HEPQEQPGVL PPEPPDVGSS DPLSMVEPLQ GRSPSYAS
Tag N-terminal His tag
Buffer Prior to lyophilization: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Fibroblast Growth Factor 21 (FGF21)
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