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Gilthead Seabream Insulin Like Growth Factor 1 (IGF1) Protein (Active)
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Catalogue No: abx260526
Price:
US$261.00
(Size: 10 µg)
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Gilthead Seabream Insulin Like Growth Factor 1 (IGF1) Protein is a recombinant cytokine expressed in E. coli, consisting of a single, non-glycosylated polypeptide chain containing 68 amino acids. IGF1 quantification was carried out by two independent methods:
Research Articles on Insulin Like Growth Factor 1 (IGF1)
- UV Spectroscopy at 280 nm using the absorbency value of 0.60 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0.
- RP-HPLC, using a calibrated solution of IGF1 as a reference standard.
| Target | Insulin Like Growth Factor 1 (IGF1) |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 98% (SDS-PAGE and SEC-HPLC) |
| Purification | Purified by proprietary chromatographic techniques. |
| Reconstitution | Reconstitute in sterile 0.4% NaHCO3, pH 8-9, to a concentration of at least 100 µg/ml. This stock solution can be further diluted if required. |
| Storage | Store desiccated at < -18 °C. After reconstitution, store at 4 °C for up to 1 week or aliquot and store at < -18 °C for long term storage. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles. |
| Molecular Weight | 7545.4 Da |
| Sequence Fragment | 68 AA. |
| Sequence | MSPETLCGAE LVDTLQFVCG ERGFYFSKPG YGPNARRSRG IVDECCFQSC ELRRLEMYCA PAKTSK. |
| Buffer | Prior to lyophilization: 0.02% NaHCO3. |
| Activity | Active |
| Biological Activity | Predicted PI: 7.72. Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active. |
| Concentration | Prior to lyophilization: 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |